Latest News


Mini simpozij infrastrukturnih centrov

Mini simpozij infrastrukturnih centrov Medicinske fakultete Univerze v Ljubljani, bo potekal 21. junija 2018 med 14:00 in 17:00 v srednji predavalnici na Medicinski fakulteti Univerze v Ljubljani na Korytkovi 2.

Taste of Genomics

Taste of Genomics: the 70th anniversary of Prof. Radovan Komel and 13th CFGBC Scientific Symposium will take place from June 20th-21st 2018 at the University of Ljubljana, Faculty of Medicine, Slovenia.

Systems Medicine Conference in Slovenia

Systems Medicine Conference in Slovenia: National Awareness Event,12th CFGBC Symposium and “Systems Medicine” Workshop took place from June 8th-9th 2017 at Hotel Slon, Ljubljana, Slovenia.


In silico analysis of CHO metabolism in dependence on the medium composition

Živa Rejc1,Tilen Tršelič1,Jakob Mraz2,Nikolaj Zimic3,Miha Mraz3,Miha Moškon3

1Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia,2Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia,3Faculty of Computer and Information Science, University of Ljubljana, Ljubljana, Slovenia

Chinese Hamster Ovary (CHO) cells have become prevalent in the production of recombinant proteins for clinical applications. Efforts have been devoted to the investigation of CHO cell metabolism and cell media composition to understand and achieve high protein production. Recently, wet lab experiments have been complemented with the systems biology approaches, which are based on the reconstruction and analysis of genome-scale metabolic models (GEMs). These models can provide an insight in the cell metabolism. Moreover, they can be used to find a set of perturbations either within the cell or within the growth medium which will increase the target product yield.
We used the state of the art producing CHO cell lines model, i.e. iCHO1766 [1], to analyse the effects that the perturbations of the medium have on the cell growth and on the production of recombinant proteins.  The analysis was performed with the perturbations of glucose and amino-acids in medium availability. We were able to identify the most influential as well as essential amino acids in selected CHO cell lines. The validation of obtained results was performed with the literature data.
We have demonstrated the applicability of constraint based modelling approaches based on flux balance analysis (FBA) on the optimisation of cellular medium composition to improve the production of recombinant proteins. Even though the accuracy of the state-of-the-art computational reconstructions of biological systems is still far from being perfect, computational approaches can be used to significantly reduce the amount of experimental as well as clinical work.
The research was partially supported by the scientific-research programme Pervasive Computing (P2-0359) financed by the Slovenian Research Agency in the years from 2009 to 2017 and by the basic research and application project Designed cellular logic (J1-6740) financed by the Slovenian Research Agency in the years from 2014 to 2017.
[1] Hefzi H, et al. (2016) Cell Syst 3(5), pp. 434-43.

2006 - University of Ljubljana, Faculty of Medicine, Center for Functional Genomics and Bio-chips.